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Sigma

SK-N-MC

90022302

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human nerve (supraorbital lymph node metastasis)
growth mode   Adherent
karyotype   2n = 46, Hypodiploid to pseudodiploid, abnormalities
morphology   Epithelial-like
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   metastasis
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Human neuroepithelioma, metastasis to supra-orbital area

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:20 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Reported to have moderate dopamine-β-hydroxylase activity. Formaldehyde-induced fluorescence indicative of intracellular catecholamines.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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