EMAIL THIS PAGE TO A FRIEND
Sigma

Sf 1Ep

90092702

Purchase

Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   rabbit skin
growth mode   Adherent
karyotype   2n = 42
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Virus replication studies (may be useful for viruses producing lesions in skin)

Cell Line Origin

Rabbit epidermis, cottontail

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan. Maximum of 80 passages expected. Cells become spindle shaped when confluent.

Cell Line Description

From minced ear epidermis of adult male cottontail rabbit with papillomatosis. Cells can be cultured continuously at pH 7.4 and 37°C but may also be adequately maintained at 25°C. Developed for studies of epidermis specific virus replication. Also know as Sf1Ep (NBL-11).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?