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Sigma

VH2

90102539, from Daboia russelii cardiovascular, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

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Properties

Related Categories CITES, Cell Culture, ECACC Cell Lines, S-Z
biological source   Daboia russelii cardiovascular
growth mode   Adherent
karyotype   2n = 34
morphology   Fibroblast-like
receptors   Not specified

Description

Cell Line Origin

Viper Russells heart

Cell Line Description

Derived from the heart of a normal female Russell′s viper (Daboia russelii). Unlike cell line VSW the cells do not produce C type particles.

Culture Medium

EMEM (HBSS) (M5775) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + fetal bovine serum (FBS / FCS) (Heat Inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2, i.e., seeding at 3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 28-30°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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