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Sigma

SW 1463

90112713

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human colon
growth mode   Adherent
karyotype   2n = 46, hypertriploid
morphology   Epithelial
products   Carcinoembryonic antigen (CEA)
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human Caucasian rectum adenocarcinoma

Cell Line Description

Established from a grade II mucous producing and grade III solid rectal adenocarcinoma from a 66 year old Caucasian female. The cells form brush borders and produce CEA.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11
D5S818: 13,14
D7S820: 9
THO1: 6,7
TPOX: 8,11
vWA: 16

Culture Medium

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split subconfluent cultures 1:2 using 0.25% trypsin or trypsin/EDTA; Medium change after 4-5 days. 37°C. No CO2.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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