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91021202

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Properties

Related Categories Cell Culture, ECACC Cell Lines, O-R More...
biological source   opossum kidney
growth mode   Adherent
karyotype   Not specified
morphology   Not specified
products   Not specified
receptors   Insulin, serotonin, alpha 2-adrenergic parathy. Hormone
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

American Opossum, kidney

Cell Line Description

Derived from proximal tubule of a kidney taken from an adult female opossum. Cells retain proximal tubule function. Possesses the following receptors: α 2 - adrenergic, insulin, serotonin, parathyroid hormone and atrial natriuretic factor.

Application

Kidney function studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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