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Sigma

SW 837

91031104

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human colon
growth mode   Adherent
karyotype   2n = 46, hypodiploid
morphology   Epithelial
products   Carcinoembryonic antigen (CEA)
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Tumourigenicity studies

Cell Line Origin

Human Caucasian rectum adenocarcinoma

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,9
vWA: 15,16

Culture Medium

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; No CO2; 37°C. After thawing the 1st subculture interval may be 14-18 days. Medium change after 4 days.

Cell Line Description

Established from a Grade IV adenocarcinoma of the rectum of a 53 year old Caucasian male. The cells produce CEA and electron microscopy reveals brush borders. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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