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Sigma

UMR-108

91060527

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   rat bone
growth mode   Adherent
karyotype   Not specified
morphology   Not specified
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Hormone studies

Cell Line Origin

Rat Sprague-Dawley osteosarcoma

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

A cloned derivative of a transplantable rat osteogenic sarcoma. Cells retain many characteristics of the tumour including responsiveness to parathyroid hormone, cytosol receptors for the bone resorbing steroid hormone 1,25-OH)2D3 and prostaglandins. Similar to cell line UMR-106.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
Related Products

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