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2-179-E11

92030602, from Mouse x Mouse Hybridoma, Reactivity Anti-TNF alpha, human

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   Mouse x Mouse Hybridoma
ImmunoDon   BALB/c mouse spleen
Myeloma   NS0
Reactivity   Anti-TNF alpha, human
application(s)   cell culture | mammalian: suitable
isotype   IgG1
shipped in   dry ice

Description

Cell Line Origin

Mouse NS0 x Mouse BALB/c spleen

Cell Line Description

This strongly neutralising antibody is directed against natural and recombinant human TNF alpha. It does not cross-react with TNF ß. It cross-reacts with simian TNF alpha but not with TNF alpha or ß of other species. It can be paired in a sandwich ELISA with 357-101-4. Disclaimer required.

Immunogen

Highly purified recombinant human tumour necrosis factor alpha

Application

Immunoassays, TNF alpha neutralisation, immunohistochemistry

Culture Medium

RPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS) (heat inactivated)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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