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HMVII

92042701

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Properties

Related Categories Cell Culture, D-I, ECACC Cell Lines More...
biological source   human vagina
growth mode   Adherent
karyotype   Not specified
morphology   Epithelial
products   Melanin
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human vaginal maligant melanoma

Cell Line Description

Established from a vaginal malignant melanoma of a 65 year-old female with blood type B. Cells produce melanin and can support the replication of influenza C virus.

Application

Replication of influenza C virus

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 8,14
THO1: 6,9
TPOX: 8
vWA: 16,17

Culture Medium

Ham′s F10 or RPMI 1640 + 2mM Glutamine + 10-20% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Pipette Controller from Brand
Protocols & Articles
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