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92050721

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human thyroid
growth mode   Adherent
karyotype   Aneuploid karyotype with marker chromosomes
morphology   Not specified
products   Calcitonin, Carcinoembryonic antigen (CEA)
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Tumourigenicity studies

Cell Line Origin

Human Caucasian thyroid medullary carcinoma

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 11
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9
TPOX: 8,11
vWA: 16,18

Culture Medium

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Population doubling is very slow, cells can take up to 5 days to reach 50% confluency; media change 2-3 times a week is recommen

Cell Line Description

Established from a thyroid medullary carcinoma of a 77 year old Caucasian female. Tumourigenic in nude mice.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
Related Products

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