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CESS

92110206

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Properties

Related Categories A-C, Cell Culture, ECACC Cell Lines More...
biological source   human blood
growth mode   Suspension
karyotype   Not specified
morphology   Lymphoblast
products   Surface Immunoglobulin ( Ig), MHC Class II
receptors   Fc, complement
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human lymphoblast

Cell Line Description

Derived from a patient with myelomonocytic leukaemia and subsequently exposed to Epstein Barr Virus (EBV). This line is EBV positive and therefore presumably derived from a normal B lymphocyte. This cell line is also positive for surface Ig, Fc receptor, complement receptor, surface Ia and EBNA. Responds to T cell replacement factor by increasing the number of IgG secreting cells. The cells should be handled under laboratory containment level 2.

Application

T cell replacement factor (TRF) assays, B cell differentiation studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,9
vWA: 16,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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