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Sigma

WEHI 3BD

92110401

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   mouse blood
growth mode   Semi-adherent
karyotype   Tetraploid
morphology   Granulocyte
products   Interleukin 3 (IL-3)
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Mouse BALB/c granulocytic leukaemia

Cell Line Description

Subclone of the line WEHI 3D. Cells will not respond to G-CSF or GM-CSF to differentiate in a plaque assay.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-5x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Mrs M Freshney, The Beatson Institute, Glasgow, UK

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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