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Sigma

V79-4

93010723

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   hamster lung
growth mode   Adherent
karyotype   2n = 22
morphology   Fibroblast
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Hamster Chinese lung

Cell Line Description

Derived from the lung tissue of a male Chinese hamster. The cells exhibit a high plating efficiency and a doubling time of 12-14 hours. Originally used in the discovery of X-ray induced damage and recovery in mammalian cells. This is a clone of cell line V79 (Sigma Catalogue number. 86041102).

Application

Mutagenesis and mammalian cell genetic studies

Culture Medium

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:14 i.e. seeding at 3x10,000-1x100,000 cells/cm2; 5% CO2; 37°C using 0.25% trypsin/EDTA.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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