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Sigma

WIL2.NS.6TG

93031001

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human spleen
growth mode   Suspension
karyotype   Not specified
morphology   Lymphoblast
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Immortalising antigen-stimulated human B-lymphocytes by somatic hybridisation

Cell Line Origin

Human Caucasian B lymphocyte

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 5x1,000-5x10,000 cells/ml; 5% CO2; 37°C.

Cell Line Description

A non Ig-secreting, HAT sensitive, 6-thioguanine resistant, human B lymphocyte. Derived from the parent line WIL2.NS (Sigma Catalogue number. 90112121).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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