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Sigma

TOU (TOU I-2)

93093001

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human blood
growth mode   Suspension
karyotype   Not specified
morphology   Lymphoblast
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Prof M-P Lefranc and Prof G Lefranc, Laboratoire d′Immunogénétique Moléculaire, Universite Montpellier II

Cell Line Origin

Human B lymphocyte, EBV transformed

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37°C.

Cell Line Description

Peripheral blood lymphocytes from a male Tunisian immortalised by EBV transformation. The cell line contains the following deletions, in the human immunoglobulin heavy chain constant region locus (IGH chromosome 14q32.3): deletion II (del EP1-A1-GP) Gm-Am genotype Gm b; -; -A2m2/Gm g; za; .A2m2. Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). Only one allotype is defined on the gamma 2 chain and may or may not be present; (.) indicates the absence of this allotype.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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