Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human blood
growth mode   Suspension
karyotype   Not specified
morphology   Lymphoblast
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C


Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Prof M-P Lefranc and Prof G Lefranc, Laboratoire d′Immunogénétique Moléculaire, Universite Montpellier II

Cell Line Origin

Human B cell lymphocyte, EBV transformed

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37°C.

Cell Line Description

Peripheral blood lymphocytes from a male Tunisian immortalised by EBV transformation. The cell line contains the following deletion, at the homozygous state, in the human immunoglobulin heavy chain constant region locus (IGH chromosome 14q32.3): deletion I (del G1-EP1-A1-GP-G2-G4) Gm-Am genotype Gm b; -; -A2m2/Gm b ; -; -A2m2. Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-).

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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