26/27 human cell line

IHW Number: 9251, 94022517

Synonym: 262



Related Categories Cell Culture, ECACC Cell Lines, HLA Collection More...
biological source   human blood
ethnicity   Native American, Piman
gender   female
growth mode   Suspension
karyotype   Not specified
morphology   Lymphoblastoid
application(s)   cell culture | mammalian: suitable
shipped in   dry ice


General description

Consanguineous: No

Homozygous: No

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

A: 0201 2402
Cw: 801
B: 51021 4801
HLA DRB1: 14
HLA DQB1: 03

Serological Profile

HLA A: 2 24
HLA C: 8
HLA B: 5 48
Bw4/bw6: 4 6

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.


This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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