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Sigma

111 BLCL human cell line

IHW Number: SLE, 94031620

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Properties

Related Categories Cell Culture, ECACC Cell Lines, HLA Collection More...
biological source   human blood
ethnicity   Caucasian
gender   male
growth mode   Suspension
karyotype   Not specified
morphology   Lymphoblastoid
application(s)   cell culture | mammalian: suitable
shipped in   dry ice

Description

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

Not specified

Serological Profile

HLA A: 1 3
HLA C: 7 0
HLA B: 7 8
HLA DR: 2 3
HLA DQ: 1 2

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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