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BCK human cell line

94050326, IHW Number: 9262

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Properties

Related Categories Cell Culture, ECACC Cell Lines, HLA Collection More...
biological source   human blood
ethnicity   Welsh/Greek
gender   female
growth mode   Suspension
karyotype   Not specified
morphology   Lymphoblastoid
application(s)   cell culture | mammalian: suitable
shipped in   dry ice

Description

General description

Consanguineous: No

Homozygous: No

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

A: 0201 3201
Cw: 0602 0304
B: 2708 1501
HLA DRB1: 15 11
HLA DQB1: 05 03

Serological Profile

HLA A: 2 32
HLA C: 6 10
HLA B: 2708 62
Bw4/bw6: 6

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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