from human blood, 94060809



Related Categories A-C, Cell Culture, ECACC Cell Lines More...
biological source   human blood
HRC Panel Number   1
ethnicity   UK Caucasian
gender   male
growth mode   Suspension
karyotype   Not Specified
morphology   Lymphoblastoid
application(s)   cell culture | mammalian: suitable


Cell Line Origin

UK Caucasian Male

Cell Line Description

A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.

This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.


As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.

Culture Medium

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 20% FBS / FCS (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.

Other Notes

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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