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Sigma

SK-N-AS

94092302

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human nerve (bone marrow metastasis)
growth mode   Adherent
karyotype   46
morphology   Polygonal
products   Not specified
receptors   Vimentin
application(s)   cell culture | mammalian: suitable
relevant disease(s)   metastasis
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Mrs C Helson, St Agnes Hospital, 305 North St, White Plains, New York

Application

Neuroblastoma differentiation studies

Cell Line Origin

Human neuroblastoma

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17

Culture Medium

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Derived from a female patient with neuroblastoma from the metastasis of the bone marrow. Tumourigenic in nude mice.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
Related Products

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