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Sigma

VA-ES-BJ

94092303

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human skin (bone marrow metastasis)
growth mode   Adherent
karyotype   66-68 Aneuploidic, pentasomy #7, multiple trisomy
morphology   Epithelial
products   Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
receptors   Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53
application(s)   cell culture | mammalian: suitable
relevant disease(s)   metastasis
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human skin epithelioid sarcoma

Cell Line Description

Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.

Application

Study of tumours. Tumourigenic in nude mice.

Culture Medium

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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