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Sigma

SK-N-DZ

94092305

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human nerve (bone marrow metastasis)
growth mode   Adherent
karyotype   44XX, monosomic for 10, 11, 13, 14, 19. Both copies of chromosome 2 missing in each cell.
morphology   Neuronal
products   p53, moderate MDR-1 expression
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   metastasis
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Tumour studies. MRD-1 resistance studies. Extremely sensitive to cis-retinoic acid.

Cell Line Origin

Human neuroblastoma

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 9,11
D5S818: 12
D7S820: 12,13
THO1: 6,9
TPOX: 8
vWA: 16,18

Culture Medium

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Spontaneously transformed cells from a female patient with neuroblastoma. The tumour was derived from a bone marrow metastasis and is tumourigenic in nude mice. Used in the study of MIBG uptake in the diagnosis and treatment of neuroblastoma. MIBG is a derivative of the antihypertensive agent guanethidine and is used as a radiopharmaceutical. SK-N-DZ is characterised by low saturable kinetics and predominant linear pattern of uptake of MIBG, with a poor ability to maintain high intracellular levels of the drug.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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