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Sigma

SUP-T1

95013123

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human blood
growth mode   Suspension
karyotype   92XXXY, -2, -2, -9, -9, 2inv(2*)(p21q11), +2inv(2), del(2*)(Q21), 2del(4)(q31q35), 2del (5)(p13p14), 2del(6)(q23q27), inv(14)(q11q32), t(7;9)(q34,q34), +der(9)t(7;9). Undergone Tetraploidisation
morphology   Lymphoblast
products   Not specified
receptors   CD4
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human T cell lymphoblastic Lymphoma

Cell Line Description

Derived from a Malignant pleural effusion taken from an eight year old caucasian male with T cell lymphoblastic lymphoma. These cells are E-rosette, Leu 5, OKT9 and CALLA negative. They also express β2M, Leu 1, 2, 3a, 4, 6, 9 and OKT10 antigens and are CD4 positive. These cells are negative for Tac, HLA-DR and surface immunoglobulin.

Application

Cells have been used for growth of HIV-1

SUP-T1 has been used in a study targeting B-cell neoplasia with T-cell receptors recognizing a cluster of differentiation 20 (CD20)-derived peptide on patient-specific human leucocyte antigen (HLA). Existing appl-SUP-T1 cells have been used for growth of HIV-1.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 2x100,000 to 2x1,000,000 cells/ml 5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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