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13C4

95032701, Reactivity: Anti-Shigella-like Toxin I (SLTI)

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   Mouse x Mouse Hybridoma
ImmunoDon   BALB/c mouse spleen
Myeloma   Sp2/0 Ag-14
Reactivity   Anti-Shigella-like Toxin I (SLTI)
application(s)   cell culture | mammalian: suitable
isotype   IgG1
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Mouse Sp2/0 Ag-14 x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against Shigella toxin and neutralises Shigella toxin activities from Shigella dysenteriae Type 1 and S. flexneri. It also reacts with the ß subunit of SLTI and neutralises the Shigella-like activities of E.coli, Vibrio cholerae, V.parahaemolyticus and Salmonella typhimurium.

Immunogen

Purified Shigella-like toxin from E.coli H30

Application

Study of shiga toxins.

Culture Medium

DMEM + 2mM glutamine + 0.15mg/ml oxaloacetate + 0.2U/ml Bovine Insulin + 0.05mg Sodium Pyruvate (NaP) + 5-10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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