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Sigma

TK6

95111735

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human lymph
growth mode   Suspension
karyotype   Not specified
morphology   Not specified
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human lymphoblast, thymidine kinase heterozygote

Cell Line Description

The thymidine kinase heterozygote cell line TK6 has been isolated from the lymphoblastoid line HH4. HH4 was derived from the WIL-2 cell line. TK6 cells are CD19 positive, 50% of the cell population express CD20 and a small sub-population has been tested positive for CD22. We cannot confirm the BVDV status of this cell line.

Application

Bromodeoxyuridine resistant assays

TK-6 cell line has been used to study the in vitro genotoxic effects of trans- and cis-2-ethylhexyl 4-methoxycinnamate (EHMC). It has also been used to study the interaction of silver nanomaterials with the cells.

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Horse Serum (HS) or FBS.

Subculture Routine

Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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