Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human intestine
growth mode   Semi-adherent aggregates
karyotype   Pseudodiploid. Modal chromosome number is 46 at 70% with polyploidy at 16.4%. The del(3) (p23), t(4,?), t(8;?) (p22), del (9) (p13), del (11) (p13), t(1;8) (p13;p24), t(1;?) (q11,?) and 2 other marker chromosomes, if any, occur only sporadically.
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C


Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Human Colorectal Carcinoma

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.0mM sodium pyruvate

Subculture Routine

Split sub-confluent cultures (70-80 %) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37 C; 5% CO2.

Cell Line Description

This cell line was derived from a fourth passage nude mouse xenograft of tumour fragments obtained from a 43 year old Asian female patient prior to therapy. The cells have a high concentration of receptors for EGF. This cell line has lost both chromosomes 8 and 11. This is one of 14 colorectal carcinoma cell lines derived by JG Park and associates during the years 1982 to 1985. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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