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Sigma

UM-UC-3

96020936

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human bladder
growth mode   Adherent
karyotype   Hypertriploid. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency.
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human Bladder Transitional Cell Carcinoma

Cell Line Description

Derived from a urinary bladder transitional cell carcinoma from a human male. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously. Hypertriploid. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t (1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) and others. The X and N3 had single copy per cell, and others were generally two or three copies per cell.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8
D16S539: 8,9
D5S818: 12
D7S820: 8,9
THO1: 6,9
TPOX: 10
vWA: 17

Culture Medium

EMEM EBSS + 2mM Glutamine + 0.1mM Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) + 1.5g/L sodium bicarbonate + 1.0mM sodium pyruvate.

Subculture Routine

Split sub-confluent cultures (70-80 %) 1:4 to 1:10 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37 C; 5% CO2.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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