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Sigma

F4N (MEL)

96121718

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Properties

Related Categories Cell Culture, D-I, ECACC Cell Lines More...
biological source   mouse unknown/unspecified
growth mode   Suspension
karyotype   Aneuploid
morphology   Lymphoblastoid
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Application

Apoptosis and DNA fragmentation studies.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Mouse Erythroleukemia

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells / ml; 5% CO2; 37°C.

Cell Line Description

The mouse erythroleukemia cell line F4N(MEL) was established in 1977. They lost the ability for erythroid differentiation by induction with DMSO or other reagents. Eight percent of the total number of cells in culture undergo spontaneous apoptosis.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
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