• USA Home
  • CHP1 - Imprint® Chromatin Immunoprecipitation Kit

EMAIL THIS PAGE TO A FRIEND
CHP1 Sigma-Aldrich

Imprint® Chromatin Immunoprecipitation Kit

Complete ChIP reaction in 6 hours in flexible strip well format

  •  NACRES NA.54

Purchase

Description

General description

Imprint Chromatin Immunoprecipitation Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification and an integrated protocol for ChIP DNA amplification with our GenomePlex® Whole Genome Amplification Kit (WGA2) . The flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or tissue in a convenient strip-well format.

For Frequently Asked Questions about this kit, please see ChIP Troubleshooting Questions.

Application

The Imprint Chromatin Immunoprecipitation Kit is a flexible, rapid and complete method for investigating protein-DNA interactions by chromatin immunoprecipitation (ChIP).
• Suitable for downstream applications
• Individual target characterization to genome-wide profiling techniques
• Characterization of signal transduction pathways
• Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

• Fast—Total protocol time of less than 6 hours making the Imprint kit the fastest on the market
• Sensitive—As few as 10,000 cells required for each ChIP sample
• Convenient—Fewest steps of any available ChIP protocol
• Flexible—Protocols for cells or tissue, and convenient strip-well format for high-throughput applications
• Complete—Includes columns and reagents for DNA purification as well as an integrated protocol for amplification with the GenomePlex technology

Legal Information

GenomePlex is a registered trademark of Takara Bio USA, Inc.

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

Kit component also available separately

Description

Product #

Add to Cart

Column Preparation Solution SDS C2112
Proteinase K from Tritirachium album, buffered aqueous glycerol solution, for molecular biology, ≥800 units/mL SDS P4850
Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution SDS P8340
Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Central nervous system
RIDADR 
UN 3316 9
WGK Germany 
WGK 3
Flash Point(F) 
53.6 °F - closed cup
Flash Point(C) 
12 °C - closed cup

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Chromatin Immunoprecipitation (ChIP) Overview

Chromatin immunoprecipitation (ChIP) assays are used to evaluate transcription factor-DNA interactions and are critical for advancing gene expression regulation and epigenetic modifications studies. ...
Keywords: Amplification, Buffers, Cell disruption, Chromatin immunoprecipitation, DNA microarrays, DNA purification, DNA replication, Digestions, Gene expression, Immunoprecipitation, Individual protein Immunoprecipitation, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, Sample preparations, Sequencing, Sonication, Transcription

Histone Modification and Chromatin Remodeling

Gene expression is governed by complex mechanisms including transcription factor binding to DNA and coordinated changes in chromatin structure. The primary protein components of chromatin are the his...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 10–16
Keywords: Acetylations, Amplification, Cancer, Cell culture, Chromatin immunoprecipitation, Cloning, DNA purification, Diseases, Epigenetics, Gene expression, Immunoprecipitation, Indicators, Methylations, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sequencing, Transcription, Whole genome amplification

Protocols

Chromatin Immunoprecipitation Kit CHP1 Protocol

Product Description Storage/Stability Workflow Preparation Instructions Procedure Analysis Troubleshooting Guide Frequently Asked Questions Materials References Poster & Application Note
Keywords: AGE, Amplification, Cell culture, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, DNA replication, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Genomics, Homogenization, Immunoprecipitation, Individual protein Immunoprecipitation, Molecular biology, Molecular probes, Nucleic acid annealing, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, Recombination, Sample preparations, Size-exclusion chromatography, Sonication, Transcription, Transduction, Whole genome amplification

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

MOD50 Imprint® DNA Modification Kit, For bisulfite DNA conversion & purification
MDQ1 Imprint® Methylated DNA Quantification Kit, To measure global DNA methylation shifts from as low as 10 ng DNA
S6576 ChIP Next Gen Seq Sepharose®
CHP2 Imprint® Ultra Chromatin Immunoprecipitation Kit, Complete ChIP kit for sensitivity, compatibility with Next-Gen sequencing
CHP2NC Imprint® Ultra Chromatin Immunoprecipitation Kit, Without Controls, ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?