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CHP2NC Sigma-Aldrich

Imprint® Ultra Chromatin Immunoprecipitation Kit, Without Controls

ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing

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Description

General description

The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 106 cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 108 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.

Application

• Suitable for downstream applications
• Individual target characterization to genome-wide profiling techniques
• Characterization of signal transduction pathways
• Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

• Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
• Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 106
• Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 106 cells
• Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.

Legal Information

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

Kit component also available separately

Description

Product #

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ChIP Next Gen Seq Sepharose® SDS S6576
IGEPAL® CA-630, for molecular biology SDS I8896
Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water    
Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture SDS S5150
Water, Nuclease-Free Water, for Molecular Biology SDS W4502
IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder SDS I5006
IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder SDS I5381
Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis) SDS A7638
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC) SDS A8456
Deoxyribonucleic acid, single stranded from salmon testes, For hybridization SDS D9156
Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution SDS M7023
Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution    
Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution SDS P8340
Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) SDS R4642
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Chromatin Immunoprecipitation (ChIP) Overview

Chromatin immunoprecipitation (ChIP) assays are used to evaluate transcription factor-DNA interactions and are critical for advancing gene expression regulation and epigenetic modifications studies. ...
Keywords: Amplification, Buffers, Cell disruption, Chromatin immunoprecipitation, DNA microarrays, DNA purification, DNA replication, Digestions, Gene expression, Immunoprecipitation, Individual protein Immunoprecipitation, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, Sample preparations, Sequencing, Sonication, Transcription

Histone Modification and Chromatin Remodeling

Gene expression is governed by complex mechanisms including transcription factor binding to DNA and coordinated changes in chromatin structure. The primary protein components of chromatin are the his...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 10–16
Keywords: Acetylations, Amplification, Cancer, Cell culture, Chromatin immunoprecipitation, Cloning, DNA purification, Diseases, Epigenetics, Gene expression, Immunoprecipitation, Indicators, Methylations, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sequencing, Transcription, Whole genome amplification

Peer-Reviewed Papers
15

References

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CHP1 Imprint® Chromatin Immunoprecipitation Kit, Complete ChIP reaction in 6 hours in flexible strip well format
CHP2 Imprint® Ultra Chromatin Immunoprecipitation Kit, Complete ChIP kit for sensitivity, compatibility with Next-Gen sequencing CHP2 kit that contains controls.

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