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CLL1136 Sigma-Aldrich

U2OS Cells GFP-NUP98

  •  NACRES NA.81

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Properties

Quality Level   200
biological source   human female bone (Source Disease: Bone osteosarcoma)
OMIM accession no.   601021
storage temp.   −196°C
Gene Information   human ... NUP98(4928)

Description

General description

U2OS GFP-NUP98 are bone osteosarcoma cells from a human 15 year old caucasian female having a ZFN modification creating a GFP-NUP98 transgene expressed from the endogenous NUP98 gene locus.

This cell line was derived from ATCC Catalog No. HTB-96.

Application

U2OS Cells have been used to assess cell proliferation using Alamar Blue and live cell imaging.

Features and Benefits

Zinc finger nuclease (ZFN)-mediated targeted integration of a fluorescent GFP tag just behind the start codon of the NUP98 gene on chromosome 11p15.4 to create a cell line exhibiting stable expression of the transgene tagged with GFP on the N-terminus of the protein.

The U2OS cells are adherent, with a doubling time of approx. 29 hours.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Preparation Note

Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3-1:6

The base medium for this cell line is McCoy′s 5A Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.

Cell freezing medium-DMSO 1X, Cat. No. C6164.

Legal Information

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Safety & Documentation

Safety Information

WGK Germany 
WGK 3

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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