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CLLS1001 Sigma-Aldrich

DLD1 Cells BAX -/-

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Properties

Related Categories Cell Biology, CompoZr Cell Lines, Disease Models More...
Quality Level   200
biological source   human male colon (Source Disease: Colorectal adenocarcinoma)
OMIM accession no.   600040
storage temp.   −196°C
Gene Information   human ... BAX(581)

Description

General description

DLD1 Cells BAX -/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.

This product corresponds to ATCC Cat. No.CCL-221

Application

Media Renewal changes two to three times per week

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vail cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3 to 1:10

The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.

The cell freezing medium is DMSO 1X (Cat. No. C6164)

Features and Benefits

These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.

Zinc finger nuclease (ZFN) knock out on chromosome 19q13.3-q13.4

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Kit component also available separately

Description

Product #

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DLD1 Control Cells 1 vial    
DLD1 Cells BAX -/- 1 vial    
Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis (COA)

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