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CLLS1014 Sigma-Aldrich

A549 Cells HIF1A -/-/-

  •  NACRES NA.81

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Properties

Related Categories Cell Biology, CompoZr Cell Lines, Disease Models More...
Quality Level   200
biological source   human male lung (Source Disease: Lung Carcinoma)
OMIM accession no.   603348
storage temp.   −196°C
Gene Information   human ... HIF1a(3091)

Description

General description

A549 Cells HIF1A -/-/- are lung carcinoma, epithelial cells, from a human caucasian male (aged 58 years), with a ZFN knock out modification.

This product corresponds to ATCC Cat. No. CCL-185

Application

Media Renewal changes two to three times per week

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3 to 1:8

The base medium for this cell line is F12 Ham Medium, Cat. No. N4888. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.

The cell freezing medium is DMSO 1X (Cat. No. C6164)

Features and Benefits

These A549 cells are adherent, with a doubling time of approx. 22 hours.

Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Kit component also available separately

Description

Product #

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A549 Cells HIF1A -/-/- 1 vial    
A549 Control Cells 1 vial    
Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis (COA)

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