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CLS3814 Sigma

Corning® Ultra-Low attachment cell culture flasks

Ultra-Low attachment 75cm2 rectangular canted cell culture flask, vent cap, sterile, 24/cs

Synonym: cell culture flasks, tissue culture flasks

  •  eCl@ss 32040501



Related Categories Cell Culture Supplies, Cell Culture Surfaces, Corning, Corning Cell Culture Flasks, Corning Flasks,
material   flask canted neck
  flask rectangular
  high-density polyethylene cap (vent)
sterility   sterile
feature   graduations
packaging   case of 24
  pack of 4
mfr. no.   Corning, 3814
maximum volume   60 mL
surface area   75 cm2
working volume   15-22.5 mL
matrix active group   Ultra-Low Attachment surface treatment


Features and Benefits

• Manufactured from optically clear virgin polystyrene
• Ultra Low Attachment surface features a covalently bound hydrogel layer that effectively inhibits cellular attachment and minimizes protein absorption, enzyme activation, and cellular activation
• Surface is non-cytotoxic, biologically inert, and nondegradable
• Lot numbers for ease in traceability
• 100% integrity tested
• Sterilized by γ-irradiation and certified nonpyrogenic

General description

The Corning Ultra Low Attachment Surface is a covalently bound hydrogel layer that is hydrophilic and neutrally charged. Since proteins and other biomolecules passively adsorb to surfaces through hydrophobic and ionic interactions, this hydrogel surface naturally inhibits nonspecific immobilization via these forces, thus inhibiting subsequent cell attachment.

This Ultra Low Attachment Surface has been shown to successfully inhibit attachment of anchorage dependent MDCK, VERO, and C6 cells grown for a period of time equal to that necessary to obtain confluent cell growth on the control surface (standard tissue culture treated polystyrene). This surface has also been shown to prevent the attachment and activation of macrophages and neutrophils.

Vent caps contain a 0.2 μm non-wettable membrane sealed to the cap, providing consistent, sterile gas exchange while minimizing the risk of contamination. These caps are highly recommended for use in all CO2 incubators, especially for long-term use. Canted neck flasks allow easier pouring and improved access to the flask for pipetting or scraping. Rectangular flasks have a ramp from the bottom to the canted neck for easier pouring and pipette access and have an anti-tip skirt to enhance stability. Surface is non-cytotoxic, biologically inert, and non-degradable.

Legal Information

Corning is a registered trademark of Corning, Inc.

Safety & Documentation

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