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CMC0003 Sigma-Aldrich

SIG10 HIGH Electrocompetent Cells

for protein expression and DNA plasmid production

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Properties

Related Categories Cloning and Expression, Molecular Biology, Transformation of Bacteria More...
grade   for molecular biology
form   buffered aqueous solution
cell transformation   competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg
shipped in   dry ice
storage temp.   −70°C

Description

Components

• SIG10 HIGH electrocompetent cells
• pUC 19 transformation control DNA
• recovery medium for cloning

Features and Benefits

• share the most useful genetic elements of standard cloning strains like DH5a, DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
• incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability.
• provide solutions for a wide range of applications at economical prices.
• Blue - white screening

General description

The SIG10 HIGH Electrocompetent Cells are high efficiency cells with a substantially lower cost per reaction and are the most economical choice for standard cloning and library construction.

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9

Documents

Certificate of Analysis

Protocols & Articles

Articles

Competent Cell Selection & E. coli Markers Guide

We offer a range of Escherichia coli bacterial cells made competent with the highest efficiencies by optimized procedure specific to each strain. Choose from 24 new competent cells for a wide variety...
Keywords: Bacterial conjugations, Cloning, Gene expression, Methylations, Recombination, Transcription, transformation

Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and pr...
Keywords: Cell culture, Cell signaling, Cloning, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction - quantitative, Protein electrophoresis, Purification, Transfection, Western blot, transformation

Protocols

SIG10 Electrocompetent Cells

Transformation is carried out in a 0.1 cm gap cuvette using 25 μl of SIG10 Electrocompetent Cells. Optimal settings for electroporation are listed in the table below. Note the alternate settings resu...
Keywords: Antibiotics, Buffers, Peptide synthesis, Purification, transformation

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

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L2897 LB Broth with agar (Lennox), Powder microbial growth medium
L5542 LB Agar Plates, pre-poured agar plates for E. coli
CMC0006 SIG10 ULTRA Electrocompetent Cells, for BAC & cosmid cloning
CMR0002 Recovery Medium for Cloning, for use with competent cells

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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