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CMC0014 Sigma-Aldrich

BL21(DE3) Chemically Competent Cells

for protein expression

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Properties

Related Categories Cloning and Expression, Molecular Biology, Transformation of Bacteria More...
grade   for molecular biology
cell transformation   competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg
shipped in   dry ice
storage temp.   −70°C

Description

Components

• BL21(DE3) chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for expression

Application

The E. coli strain BL21 (DE3) has been used to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.

Features and Benefits

• a transformation efficiency of >1 × 107 cfu/μg.
• economical prices
• offering superior value for everyday protein expression work.

General description

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9

Documents

Certificate of Analysis

Certificate of Origin


Same Products - New Packaging
Protocols & Articles

Articles

Competent Cell Selection & E. coli Markers Guide

We offer a range of Escherichia coli bacterial cells made competent with the highest efficiencies by optimized procedure specific to each strain. Choose from 24 new competent cells for a wide variety...
Keywords: Bacterial conjugations, Cloning, Gene expression, Methylations, Recombination, Transcription, transformation

Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and pr...
Keywords: Cell culture, Cell signaling, Cloning, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction - quantitative, Protein electrophoresis, Purification, Transfection, Western blot, transformation

Protocols

BL21 Chemically Competent Cells

BL21 Chemically Competent Cells are transformed in 40 μL reactions. Cells are packaged with sufficient volumes for 1, 2, or 4 reactions per tube.
Keywords: Antibiotics, Centrifugation, Gene expression, transformation

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Peer-Reviewed Papers
15

References

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L3022 LB Broth (Lennox), Powder microbial growth medium
L2897 LB Broth with agar (Lennox), Powder microbial growth medium
L5542 LB Agar Plates, pre-poured agar plates for E. coli
CMC0015 BL21(DE3) pLysE Chemically Competent Cells, for protein expression
CMC0017 OverExpress C41(DE3) Chemically Competent Cells, for the highest protein expression
CMC0023 CONTROLLER SIG10 Chemically Competent Cells, for controlled protein expression
CMC0024 CONTROLLER BL21(DE3) Chemically Competent Cells, for controlled protein expression
CMR0001 Recovery Medium for Expression, for use with competent cells

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