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CMC0017 Sigma-Aldrich

OverExpress C41(DE3) Chemically Competent Cells

for the highest protein expression

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Properties

Related Categories Cloning and Expression, Molecular Biology, Transformation of Bacteria More...
grade   for molecular biology
cell transformation   competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg
shipped in   dry ice
storage temp.   −70°C

Description

General description

OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.

Application

OverExpress C41 (DE3) chemically competent cells have been used for expression screening.

Features and Benefits

• Express genes cloned into any T7 vector with these BL21(DE3) derivatives
• Effective in expressing toxic & membrane proteins
• Cited in over 350 research articles

Components

• OverExpress C41(DE3) chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for expression


Legal Information

OverExpress is a trademark of Imaxio, S.A.

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Competent Cell Selection & E. coli Markers Guide

We offer a range of Escherichia coli bacterial cells made competent with the highest efficiencies by optimized procedure specific to each strain. Choose from 24 new competent cells for a wide variety...
Keywords: Bacterial conjugations, Cloning, Gene expression, Methylations, Recombination, Transcription, transformation

Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and pr...
Keywords: Cell culture, Cell signaling, Cloning, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction - quantitative, Protein electrophoresis, Purification, Transfection, Western blot, transformation

Protocols

OverExpress™ Chemically Competent Cells

OverExpress™ Chemically Competent Cells are provided in aliquots of 40 μL sufficient for one transformation reaction.
Keywords: Antibiotics, Centrifugation, Gene expression, transformation

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Peer-Reviewed Papers
15

References

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CMC0024 CONTROLLER BL21(DE3) Chemically Competent Cells, for controlled protein expression
CMR0001 Recovery Medium for Expression, for use with competent cells

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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