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CN304-05 Sigma-Aldrich

Canine Aortic Endothelial Cells: CnAOEC (Cryovial)

  •  NACRES NA.81

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Properties

Related Categories Cell Culture, Endothelial Cells, Primary Cell Culture, Primary Cells
biological source   canine aorta
packaging   pkg of 500,000 cells
growth mode   Adherent
karyotype   2n = 78
morphology   Endothelial
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cardiovascular diseases, diabetes
shipped in   dry ice
storage temp.   −196°C

Description

General description

CnAOEC provide a useful model to study cardiovascular diseases and test potential therapeutic agents, not only important from a veterinary perspective, but also having implications for human health. In a study aiming to adapt an immunomagnetic isolation protocol used in human patients to isolate circulating endothelial cells in dogs, CnAOEC cells were shown to be positive for von Willebrand factor, CD146 and stained with Ulex europaeus agglutinin 1 (Wills, 2009). To investigate the mechanism of N-terminal portion of pro C-type natriuretic peptide (NT-pCNP), a sepsis biomarker in humans and dogs, CnAOEC were treated with a variety of agents and it was found that lipopolysaccharide, TNF-α and IL-1β, but not IL-6, IL-10, IL-21, CXCL-8, IFN-γ, VEGF-A, stimulated NT-pCNP production (Osterbur, 2012, 2013). CnAOEC were also used to demonstrate vasoprotective activity of pomegranate and soy isoflavons in order to prevent endothelial disfunction in dogs which commonly leads to cardiovascular disease (Baumgartner-Parzer, 2012). Finally, CnAOEC were utilized as a control to compare the growth of normal canine endothelial cells and aggressive canine hemangiosarcoma cells in a study investigating signaling pathways underlying hemangiosarcoma oncogenesis (Murai, 2012), and as a VEGFR2-positive control in a study aimed at understanding the reasons for reduced numbers of circulating progenitor cells in cardiovascular disease (Boilson, 2010).

Cell Line Origin

Aorta

Application

cardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testing

Components

Canine Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Canine Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings

Subculture Routine

Please refer to the CnAOEC Culture Protocol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Protocol: Canine Aortic Endothelial Cells Culture

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