• USA Home
  • CORET - PCR Core kit with Taq DNA polymerase

EMAIL THIS PAGE TO A FRIEND
CORET Sigma

PCR Core kit with Taq DNA polymerase

PCR components in separate tubes to allow for optimization

Purchase

Properties

Related Categories Molecular Biology, PCR/Amplification, Routine PCR Amplification More...
form   liquid
feature   hotstart: no
color   colorless
shipped in   wet ice
storage temp.   −20°C

Description

Application

This kit contains all the reagents necessary to amplify genomic DNA, cDNA, or cloned DNA templates. Each component has been tested in the Polymerase Chain Reaction. Optimal concentrations of Taq polymerase, template DNA, primers, and MgCl2 will depend on the specifics of the system. It may be necessary to optimize each component separately, especially the Taq and Mg2+ concentrations and the cycling parameters.

Other Notes

The following reagents are required but not provided in the kit:
• Mineral Oil, M 8662 (optional)
• Thermocycler
• Primers
• DNA to be amplified
• Chloroform, C 7559 (optional)

Quantity

Kit contains enough reagents for 250 units of Taq DNA polymerase.

Legal Information

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Price and Availability


Your Key to Success

PCR Selection Tool

Kapa Biosystems

Kit component also available separately

Description

Product #

Add to Cart

10X PCR Buffer, Optimized for routine PCR with MgCl2 included 1 mL/vial SDS P2192
10X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2 1 mL/vial SDS P2317
Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 1 mL/vial SDS M8787
Water, PCR Reagent 3 vials SDS W1754
Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .25 mL SDS D7295

Kit component only

Description

Product #

Add to Cart

Taq DNA polymerase 250 U SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?