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CRISPR Sigma-Aldrich

Custom CRISPR Products

Synonym: CRISPR, CRISPRs, Cas9, Crispr RNA, Crispr/Cas System, Synthetic CRISPR, crRNA, tracrRNA

 Visit the Functional Genomics learning center to read and learn more about our entire gene knockdown, knockout and expression portfolio 
Learning Center

Sanger CRISPR QuickPick Knockout Clones

  • MilliporeSigma and The Wellcome Trust Sanger Institute have partnered to create the world’s first whole genome arrayed CRISPR libraries. Together, the combined libraries contain over 74,000 individual CRISPR clones designed to maximize knockout of nearly every protein-coding gene in the human and mouse genomes. These ready-to-use constructs are now available to the global research community as individual clones, gene panels, and whole genome libraries.
  • Use the Ordering icon to the right to order human or mouse Sanger CRISPR clones in any one of three formats to knockout your gene of interest.
    Use the links below for specific information on each format:
    Bacterial glycerol stocks
    Transfection-grade plasmid DNA
    Lentiviral transduction particles

Custom CRISPR Clones

Take control of your CRISPR genome editing and screening experiments with the CRISPR design tool. Browse dozens of genomes to design and order custom gRNAs ready for use with any of our available Cas9 products.

Design CRISPR Targets

  • Click on the SEARCH button to open the CRISPR Design Tool
  • Select your desired genome and choose your genes of interest
  • Set parameters for the designs
  • View and export a list of ranked gRNAs
  • Order your custom gRNAs

Order CRISPR Targets

Cas9 mRNA, Expression Plasmids, and Recombinant Protein

Recommended Uses: Microinjection, transient transfection, genome editing (knockout/knock-in) in cell culture (mRNA); transient transfection, genome editing in cell culture (knockout/knock-in), FACS enrichment (plasmid)

  • Codon-optimized Cas9 is available in both wild type and Cas9-D10A nickase versions.
  • Transfect Cas9 expression vectors with optional fluorophores for FACS enrichment and easy visualization of transfection efficiency.
  • Microinject either purified, injection-ready Cas9 protein or mRNA.
  • Ready-to-ship in 2 business days.
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CRISPR Positive Controls

Recommended Uses: Validate the efficiency of your genome editing experiment or screen

  • Verify the success of your genome editing experiments with validated guide RNAs against the human EMX1 gene
  • Positive controls are available for both the wild type and nickase CRISPR systems
  • Wild type Cas9 or Cas9-D10A nicking enzyme is provided with the purchase of the corresponding control
  • Ready-to-ship in 2 business days
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CRISPR Universal Negative Controls

Recommended Uses: Confirm the veracity of your genome editing experiment or screen

  • Universal Negative Controls express a guide RNA that has been bioinformatically designed not to target any sequence in the human, mouse, or rat genomes.
  • Enables the expression of Cas9 and GFP without targeting a DNA locus.
  • Universal Negative Controls are provided in the all-in-one CRISPR vector expressing both Cas9 and a non-targeting guide RNA.
  • For most CRISPR experiments, the Universal Negative Control 1 is recommended; Universal Negative Control 2 and 3 can be used as additional controls or as alternatives to Universal Negative Control 1.
  • Ready-to-ship in 2 business days.
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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?