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CS0004 Sigma-Aldrich

Nuclease micrococcal from Staphylococcus aureus

With reaction buffer and BSA solution

Synonym: Endonuclease micrococcal, MNase, Micrococcal endonuclease, Nuclease micrococcal from Staphylococcus aureus

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Properties

shipped in   wet ice
storage temp.   −20°C

Description

General description

Nuclease Micrococcal (MNase) is the extracellular nuclease of Staphylococcus aureus. The enzyme is an endo-exonuclease .This Endo-exonuclease preferentially digests single-stranded nucleic acids. Thus, nuclease micrococcal activity results in the production of mononucleotides and oligonucleotides with terminal 3′-phosphates. Nuclease Micrococcal is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.
Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes

Application

• Chromatin Immuno-Percipitation (ChIP) analysis
• Nucleosome preparation
• Removal of nucleic acids present in protein preparation
• Reduction of viscosity of cell lysates during non-mechanical cell lysis preparation
• RNA sequencing and transcriptomic profiling

Biochem/physiol Actions

Hydrolyzes 5′-phosphodiester bonds of DNA.

Other Notes

Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

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