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CS0410 Sigma-Aldrich

Glutathione-S-Transferase (GST) Assay Kit

sufficient for 500 multiwell tests

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Properties

Related Categories Cell Analysis, Cell Biology More...
usage   sufficient for 500 multiwell tests
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GSTA4(2941), GSTM1(2944)

Description

Application

The Glutathione S-Transferase Assay kit utilizes 1-Chloro-2,4-dinitrobenzene (CDNB), which is suitable for the broadest range of GST isozymes. Upon conjugation of the thiol group of glutathione to the CDNB substrate, there is an increase in the absorbance at 340 nm.

Biochem/physiol Actions

Glutathione S-transferases (GSTs) are a group of enzymes important in the detoxification of many xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity is present in plants, insects, yeast, bacteria, and most mammalian tissues especially in the liver, which plays a key role in detoxification.

General description

The Glutathione S-Transferase (GST) Assay Kit is designed to measure total GSTactivity. It can be used to measure GST activity in cell and bacterial lysates, tissue homogenates, and in plasma
and erythrocyte lysates

Kit component also available separately

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Dulbecco's Phosphate Buffered Saline 100 mL    
L-Glutathione Reduced 1 g    

Kit component only

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GST (Control) .1 mL    
Sample Buffer 5 mL    
Substrate (CDNB) 1.2 mL    
Safety & Documentation

Safety Information

Signal word 
Danger
RIDADR 
UN 3316 9
WGK Germany 
2
RTECS 
CZ0525000
Flash Point(F) 
381.2 °F
Flash Point(C) 
194 °C

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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06693 Timestrip Plus -20 °C

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