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CS0800 Sigma-Aldrich

Cathepsin D Assay Kit

sufficient for 120 assays (100 μL)

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Properties

Related Categories Apoptosis and Cell Cycle, Cathepsins and Granzymes, Cathepsins and Granzymes Enzymes, Cell Analysis, Cell Biology,
usage   sufficient for 120 assays (100 μL)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CTSD(1509)

Description

Biochem/physiol Actions

Cathepsin D, a ubiquitous aspartic protease belonging to the A1 peptidase family, is found intracellularly in lysosomes. The enzyme has been associated with various biological processes such as: apoptotic events (e.g., the release of cytochrome c from mitochondria and the loss of the transmembrane potential (ΔΨ), aging, Alzheimer′s disease, and breast cancer.

Features and Benefits

• Contains all the reagents required for the measurement of the cathepsin D activity in cell and tissue lysates.
• The bottom limit of detection is 750 pg of protein (or 13 femtomoles of enzyme).
• A single step reaction with direct, real time observation of the degradation product released by the enzyme.
• The kit has been tested on extracts from CHO, HeLa, HEK 293, A431, HepG2, U937, and Jurkat cells on S. cerevisiae and on rat brain, kidney and liver tissues.

Analysis Note

The assay is based on the hydrolysis, by the enzyme, of an internally quenched fluorimetric substrate.

Kit component only

Description

Product #

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Assay Buffer 1x 20 mL    
Cathepsin D from bovine spleen 5 U    
Albumin Solution 1 mL    
Cathepsin D Substrate Solution .25 mL    
7-Methoxycoumarin 4-acetic acid Standard Solution .1 mL    
Pepstatin A Inhibitor Solution .6 mL    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3265 8 / PGIII

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

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Product #

Description

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C3138 Cathepsin D from bovine spleen, lyophilized powder, ≥2.0 units/mg protein
C8696 Cathepsin D from human liver, lyophilized powder, ≥250 units/mg protein (E1%/280)
C0715 Monoclonal Anti-Cathepsin D antibody produced in mouse, clone CTD-19, ascites fluid

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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