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CS1070 Sigma-Aldrich

Transglutaminase Assay Kit

sufficient for assays in two 96-well plates

Synonym: TGases Assay Kit

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Properties

Related Categories Apoptosis Detection, Apoptosis Detection Kits, Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience More...
Quality Level   200
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TGM1(7051), TGM2(7052), TGM3(7053), TGM4(7047), TGM5(9333), TGM6(343641), TGM7(116179)

Description

General description

The Transglutaminase Assay Kit is designed for the detection of transglutaminase activity in biological samples. The kit can also be used for screening of transglutaminase inhibitors/activators.

Application

The assay is based on transglutaminase catalysis of a covalent bond formation between the free amine group of Poly L-Lysine, which is covalently attached to the plate surface, and the γ-carboxamide group of biotin-TVQQEL-OH substrate present in the assay buffer. This reaction immobilizes biotin to the plate surface. The amount of immobilized biotin is proportional to the amount of active transglutaminase in the sample. The amount of immobilized biotin is determined using streptavidin-peroxidase and TMB substrate.

Biochem/physiol Actions

Transglutaminases (TGases) are a widely distributed and unique group of calcium dependent enzymes that catalyze the post-translational modification of proteins by the formation of isopeptide bonds. This occurs either through protein cross-linking via formation of γ-glutamyl-ε-lysine bonds or through incorporation of primary amines at selected peptide-bound glutamine residues. The cross-linked products, often of high molecular mass, are highly resistant to mechanical challenge and proteolytic degradation, and their accumulation is found in a number of tissues and processes, where such properties are important including skin, hair, blood clotting, and wound healing. Deregulation of the enzyme activity contributes to a number of human diseases.

Other Notes

The detection limit is 0.003 mU/assay or 0.03mU/ml of Transglutaminase.

Kit component also available separately

Description

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Transglutaminase from guinea pig liver, lyophilized powder, ≥1.5 units/mg protein 2 ea SDS T5398
3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, peroxidase substrate SDS T0440
Streptavidin−Peroxidase from Streptomyces avidinii, lyophilized powder 100 μg SDS S5512
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3316 9
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers
15

References

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