• USA Home
  • CS1070 - Transglutaminase Assay Kit

CS1070 Sigma-Aldrich

Transglutaminase Assay Kit

sufficient for assays in two 96-well plates

Synonym: TGases Assay Kit



Related Categories Apoptosis Detection, Apoptosis Detection Kits, Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience More...
Quality Level   200
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TGM1(7051), TGM2(7052), TGM3(7053), TGM4(7047), TGM5(9333), TGM6(343641), TGM7(116179)


General description

The Transglutaminase Assay Kit is designed for the detection of transglutaminase activity in biological samples. The kit can also be used for screening of transglutaminase inhibitors/activators.


The assay is based on transglutaminase catalysis of a covalent bond formation between the free amine group of Poly L-Lysine, which is covalently attached to the plate surface, and the γ-carboxamide group of biotin-TVQQEL-OH substrate present in the assay buffer. This reaction immobilizes biotin to the plate surface. The amount of immobilized biotin is proportional to the amount of active transglutaminase in the sample. The amount of immobilized biotin is determined using streptavidin-peroxidase and TMB substrate.

Biochem/physiol Actions

Transglutaminases (TGases) are a widely distributed and unique group of calcium dependent enzymes that catalyze the post-translational modification of proteins by the formation of isopeptide bonds. This occurs either through protein cross-linking via formation of γ-glutamyl-ε-lysine bonds or through incorporation of primary amines at selected peptide-bound glutamine residues. The cross-linked products, often of high molecular mass, are highly resistant to mechanical challenge and proteolytic degradation, and their accumulation is found in a number of tissues and processes, where such properties are important including skin, hair, blood clotting, and wound healing. Deregulation of the enzyme activity contributes to a number of human diseases.

Other Notes

The detection limit is 0.003 mU/assay or 0.03mU/ml of Transglutaminase.

Kit component also available separately


Product #

Add to Cart

Transglutaminase from guinea pig liver, lyophilized powder, ≥1.5 units/mg protein 2 ea SDS T5398
3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, peroxidase substrate SDS T0440
Streptavidin−Peroxidase from Streptomyces avidinii, lyophilized powder 100 μg SDS S5512
Safety & Documentation

Safety Information

GHS05  GHS05
Signal word 
Hazard statements 
UN 3316 9
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?