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CompoZr Custom Zinc Finger Nuclease (ZFN)

ZFN plasmid only

Synonym: CompoZr® Custom Zinc Finger Nuclease (ZFN)

Note: This is a custom product. For more information on pricing and customization options, please fill out the form below and our Technical Sales Specialist will contact you directly.



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Properties

Related Categories Custom CompoZR ZFN Services, Functional Genomics and RNAi, Molecular Biology More...
Quality Level   200
product line   CompoZr®
shipped in   dry ice
storage temp.   −70°C

Description

General description

Zinc Finger Nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Double-strand breaks are important for site-specific mutagenesis in that they stimulate the cell’s natural DNA-repair processes, namely homologous recombination and Non-Homologous End Joining (NHEJ). By implementing established, field-proven methods, these processes can be harnessed to generate precisely targeted genomic edits, resulting in cell lines with precise and heritable gene deletions, integrations or modifications.

Application

Functional Genomics/Target Validation
• Creation of gene knockouts in multiple cell lines
• Complete knockout of genes not amenable to RNAi
Cell-based Screening
• Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes
Cell Line Optimization
• Creation of cell lines that produce higher yields of proteins or antibodies

The CompoZr Custom ZFN service can be used to target genes from a variety of organisms. Validation of the custom ZFNs is completed in a proxy cell line for human, mouse, rat and hamster (CHO) projects. In this assay, the candidate ZFNs are delivered to the proxy cell line and activity is identified and measured. Activity is measured by amplifying over the ZFN target site and then using a nuclease mismatch enzyme to cut DNA strands that have been modified. ZFN efficiency can be measured based on the densitometry results of this assay. CompoZr Custom ZFN Projects for human, mouse, rat, and hamster (CHO) will have a production timeline of 10 weeks after the ZFN design is approved.

Components

• Best Performing ZFN Pair
• ZFN Pair in Plasmid Form
• Forward and Reverse Primers that allow for determination of rate of mutation and for screening of individual clones
• Positive Control DNA
• Used to determine a baseline cutting efficiency

Legal Information

All Zinc Finger Nucleases are sold under license from Sangamo Biosciences. For a copy of the Label License provided with purchase of CompoZr ZFNs, please visit the ZFN Label License page.

3D Insert is a trademark of 3D Biotek, LLC

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

CRISPR on the Brain? Standardized Functional Genomics Tools Advance Neuroscience

Deciphering the genetics of neuroscience has always been challenging. Heterogeneous tissue microenvironments, complex genetic interactions and phenotypes, lack of model systems that accurately mimic ...
Keywords: Functional genomics, Gene expression, Genetic, Genetics, Genomics, Neuroscience, Sequencing

FP-ZFNs or Fluorescent Protein-Linked Zinc Finger Nucleases

Zinc finger nucleases (ZFNs) have enabled highly efficient gene targeting in a wide variety of cell types and organisms. While CompoZr® methods for engineering highly active ZFNs have become robust a...
Keywords: Cloning, Gene expression, Methods, Microscopy, Protocols, Transfection, Type, Vectors

Peer-Reviewed Papers
15

References

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CSTZFN CompoZr® Custom Zinc Finger Nuclease (ZFN), ZFN plasmid and mRNA

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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