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CompoZr® Targeted Integration Kit - AAVS1

Transgene Integration in the Human Genome

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Properties

Related Categories CompoZr Targeted Integration Kits, Functional Genomics and RNAi, Molecular Biology More...
Quality Level   200
product line   CompoZr®
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... AAVS1(17)

Description

General description

The CompoZr Targeted Integration Kit – AAVS1 is designed to rapidly integrate a user-specified gene of interest (GOI) into the adeno-associated virus integration site 1 (AAVS1) on human chromosome 19. The Zinc Finger Nuclease (ZFN) in this kit targets the AAVS1 site with high specificity, creating a double strand break. The cell then uses the pZDonor plasmid, with homology arms to the AAVS1 genomic region, in homology-directed repair to replace the natural AAVS1 locus. The pZDonor plasmid can be engineered to contain your GOI and will direct targeted integration of your GOI into the AAVS1 locus.

Features and Benefits

•Effective integration efficiency without antibiotic selection
The ZFNs engineered to target AAVS1 are a well-validated pair that cut the DNA with high efficiency. ZFN-aided targeted integration can achieve 20% efficiency in some cell types without antibiotic selection, representing an improvement of three to four orders of magnitude in efficiency over normal homologous recombination events1.

•Rapid biallelic insertions through a single transfection
ZFN mediated homology-directed repair leads to gene addition within 48 hours of transfection of ZFN mRNA/plasmid donor, with both monoallelic and biallelic insertions1.

•Controlled transgene effects through use of single genomic locus
In the past, most transgenic cell lines have been constructed via random integration of a plasmid. This results in a collection of clones with greatly varied expression levels and expression stability. Targeted integration into the AAVS1 locus will provide a common integration site with stable, uniform expression levels1.

• Eliminates need to engineer cell lines with transgene landing pads
Transgene integration into the genome has previously been achieved via recombination through recombinases such as Cre and FLP. This requires prior insertion of a transgene landing pad into the genome through random integration followed by transgene addition, making it a lengthy two step process. Targeted integration into AAVS1 eliminates the first step, making transgene expression a simple 1-step process1.

Legal Information

All Zinc Finger Nucleases are sold under license from Sangamo Biosciences. For a copy of the Label License provided with purchase of CompoZr ZFNs, please visit the ZFN Label License page.

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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