• USA Home
  • CYP1 - CelLytic Y Plus Kit

CYP1 Sigma

CelLytic Y Plus Kit

For enzymatic yeast cell lysis




The kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris, and Schizosaccharomyces pombe yeast strains. The procedure employs spheroplast formation by enzymatic digestion of the yeast cell wall followed by cell protein extraction (with or without detergent), while avoiding protein degradation and interference with protein immuno­reactivity and biological activity.
The cell extract is compatible with:
• Reporter gene expression assays (e.g., β-galactosidase, alkaline-phosphatase)
• Immunoassays (Western blots, immunoprecipitation)
• Affinity based purification (e.g. FLAG, glutathione S- transferase (GST) and histidine-tagged fusion proteins)
• DNA-protein interaction assay (e.g., gel-shift)
• Coomassie and silver staining
The kit is suitable for spheroplast formation and protein extraction from 10-40 grams of yeast cell pellet.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Celebrate Pi Day!

Kit component only


Product #

Add to Cart

Reaction Buffer 120 mL SDS    
1 M DTT 5 mL SDS    
Lyticase 25000 U SDS    
Extraction buffer 60 mL SDS    
Triton X-100 10 mL SDS    
Protease inhibitor cocktail 2 mL SDS    
Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles


Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?