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CYTOCOX1 Sigma-Aldrich

Cytochrome c Oxidase Assay Kit

sufficient for 100 tests, soluble and membrane bound mitochondria

  •  NACRES NA.84




The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.

Biochem/physiol Actions

Cytochrome c oxidase [EC] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.

Features and Benefits

• Simple, optimized protocol - Obtain reproducible results without special training needs
• Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
• Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
• May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
• Use to analyze the intactness of mitochondrial membranes
• Simple colorimetric measurement of solution

Kit component only


Product #

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Assay Buffer 5× 25 mL    
Enzyme Dilution Buffer 2× 20 mL    
Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mg    
Cytochrome c Oxidase positive control 1 vial    
n-Dodecyl β-D-maltoside, ≥98% (GC) 10 mg    
DL-Dithiothreitol solution, 1 M .4 mL    
Safety & Documentation

Safety Information

NONH for all modes of transport
Protocols & Articles


Apoptosis Assays

Apoptosis, or programmed cell death, is a growth-limiting regulatory mechanism by which cells can trigger their own death in response to extracellular signals because of irreparable cellular or DNA d...
Keywords: Apoptosis, Autophagy, Cancer, Cell culture, Detection methods, Diseases, Enzyme-linked immunosorbent assay, Flow cytometry, Immunofluorescence, Immunohistochemistry, Microscopy, Phosphorylations

Organelle Isolation | BioFiles Volume 6, Number 5 — Centrifugation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, a...
Mark Frei
BioFiles v6 n5, 22–25
Keywords: Capture ELISA, Cell culture, Centrifugation, Diffusion, Direct immunofluorescence, Dot blot, Enzyme-linked immunosorbent assay, Flow cytometry, Immunoassay, Immunocytochemistry, Immunoelectrophoresis, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Indirect ELISA, Individual protein Immunoprecipitation, Microarray Analysis, Microscopy, Radioimmunoassay, Scanning electron microscopy, Western blot

Peer-Reviewed Papers


Related Products

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MITOISO1 Mitochondria Isolation Kit, sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues
MITOISO2 Mitochondria Isolation Kit, sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells
MITOISO3 Yeast Mitochondria Isolation Kit, sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells
GL0010 Golgi Isolation Kit, sufficient for 50 g (tissue)
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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