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D1319 Sigma-Aldrich

Anti-DAP Kinase 1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-DAPK1, Anti-DAPK, Anti-Death associated protein kinase 1

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, DA-DD,
species reactivity   human, mouse (predicted), rat (predicted)
application(s)   immunoprecipitation (IP): 2.5-5.0 μg using extracts of human HeLa cells
  western blot: 1-2 μg/mL using whole extracts of human HeLa cells
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~160 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DAPK1(1612)
mouse ... Dapk1(69635)
rat ... Dapk1(306722)
biological source   rabbit
antibody product type   primary antibodies
conjugate   unconjugated
UniProt accession no.   P53355

Description

Immunogen

synthetic peptide corresponding to amino acids 2-13 of human DAP-kinase 1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

General description

Death-associated protein kinase (DAPK) belongs to the serine/threonine kinase family that regulates apoptosis, growth factor signaling and also may be involved in modulating immune responses. DAPK1 mediates cellular functions such as membrane blebbing and autophagy and inhibits the activity of prolyl isomerase. Anti-DAPK1 antibody is specific for human DAPK1 (approx. 160 kDa). In immunoblotting, detection of the DAPK1 band is specifically inhibited with the immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-DAP Kinase 1 antibody is suitable for use in immunoprecipitation (2.5-5.0 μg using extracts of human HeLa cells) and western blot (1-2 μg/mL using whole extracts of human HeLa cells).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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D4941 Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal, clone DKPS308, purified from hybridoma cell culture
D2178 Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse, clone DAPK-55, ascites fluid

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